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href="http://www.tfyqchina.cn/index.php?case=archive&act=list&catid=52" target="_self">土壤冻干机</a>在底泥中抗生素测定的应用，包括以下步骤：</p><p> (1)样品制备：通过<a href="http://www.tfyqchina.cn" target="_self">冻干机</a>在低温真空环境下把土壤冷冻干燥，然后再研磨；</p><p> (2)提取步骤：称取一定量冻干研磨后的样品，加15mL磷酸盐缓冲盐，用NaOH 或HC1溶液调节pH至4~5后，加入同位素提取内标液;再加入20mL乙臆后，超声重复提取二 次，合并提取液，旋蒸浓缩。</p><div><div> (3)分离净化步骤:用4mL甲醇洗脱小柱两次，洗脱液收集于10mL玻璃离心管中；最 后将洗脱液氮吹浓缩(干浴温度为35°C)至恰好吹干，加入500yL含0.025%甲酸的甲醇/水 (体积比1:1)和50ng进样内标液，涡旋混合均匀，用0.22μπι的针筒过滤器过滤后，保存于自 动进样样品瓶中，作为仪器的净化试样；<br style="box-sizing: border-box;"/> (4)分析检测步骤:HPLC_MS/MS测定:使用高效液相色谱仪和质谱仪测定，色谱柱为C18液相色谱柱，3.5μπιX 3.0mmX 150mm，。电喷雾电离源(，多反应监测模式，HPLC条件为：进样体积10yL;柱温40 °C;流动相为高纯水(含0.01%的甲酸)和乙腈，流速为0.40 111171^11;通过各目标物的保留时 间、定性离子对进行定性分析;通过同位素的峰面积比值进行定量分析，得出净化试样的测 定结果。</div></div></div> </div> <div class="blank30"></div> <div class="blank30"></div> <!--自动调用自定义字段--> <div class="blank20"></div> <div class="blank30"></div> </div> <!-- 投票 --> <script src="/index.php?case=vote&act=view&aid=922"></script> <div class="w_set"> <!-- 自定义表单开始 --> <!-- 自定义表单结束 --> <div class="blank60"></div> <!-- 上下页开始 --> <div id="page"> <strong>上一篇</strong><a href="/html/news/show_921.html">土壤冻干机优势</a> <div class="blank10"></div> <strong>下一篇</strong><a href="/html/news/show_923.html">冻干机在纸质文物应用，真空冷冻干燥的作用</a> </div> <!-- 上下页结束 --> </div> <!-- 相关文章开始 --> <div class="blank60"></div> <!-- 页面标题开始 --> <div class="c_h1"> <div> <h1></h1> <p class="t_tools1">相关内容</p> </div> </div> <div class="w_set"> <ul class="list_page_1"> <!-- 内容列表开始 --> <li> <div class="list_page_date"> <h1>13</h1> <p>2018-12</p> </div> <div class="li_text_w"> <p><a title="" href="/html/news/show_922.html" target="_blank" class="list_page_t">土壤冻干机在底泥中抗生素测定的应用</a></p> <p>土壤冻干机在底泥中抗生素测定的应用，包括以下步骤：(1)样品制备：通过冻干机在低温真空环境下把土壤冷冻干燥，然后再研磨；(2)提取步骤：称取一定量冻干研磨后的样品，加15mL磷酸盐缓冲盐，用NaOH 或HC1溶液调节pH至4~5后，加入同位素提取内标液;再加入20mL乙臆后，超声… <a href="/html/news/show_922.html" target="_blank">[了解更多]</a></p> <div class="clear"></div> </div> <div class="clear"></div> </li> <li> <div 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